In the Trypan blue exclusion assay, in the presence of the extracts, the cells obtained viabilities greater than 93%, and using the kit to determine LDH enzyme activity, the percentage release of the enzyme relative to the positive control (Triton X-100) was low compared with the negative control values.
Elution of HA-pNIPAAM from the scaffold was necessary to obtain good viability.
Low viscosity alginate, lower crosslinking calcium ion concentration, and lower concentration of alginate and gelatin were found to obtain high viability of cells encapsulated in the gelatin matrix after removal of the alginate template by EDTA treatment by allowing rapid dissociation and diffusion of alginate polymers.
This assay was used to obtain the viability curves presented in Figures 1 and 2.
In general, the dose of RCAd11pGFP required to obtain 50% viability differed greatly, as 3.6 vp per cell were required for A549 and HT-29 cells, 36 vp per cell were needed for T84 cells, and 360 or more vp per cell were needed for HCT-8 and LS174T cells.
The cure rate obtained without viability testing was 43.9% in the As+SMP group versus 53% in the PZQ group (Chi2 = 6.44, p = 0.011).
Comparable results were only obtained for viability: the mean % of sperm with integral membrane was distinct in the three groups (64.6±3.5 for donors, 44.9±5.5 for normozoospermic and 19.5±6.5 for OATs; Ps <0.05).
After subtracting this absorbance value, we obtained the viability of the neuronal subpopulation.
Absorbance was read at 405 nm and the enzyme activity was normalized to cell number obtained from viability assays.
For functionality test, samples desiccated for 15 minutes were chosen, in view of optimal cell number obtained from viability assay.
