The obtained turnover number of the immobilized lipase was almost the same as that of the free lipase in the batch experiment, while the Michaelis constant of the immobilized lipase was greater than that in the batch experiment by a factor of 3. A mathematical model was also employed to interpret the results of microreactor experiments performed under various conditions.
We obtained turnover numbers from enzyme database Brenda [ 28], enzyme masses from MetaCyc [ 27].
However, to obtain 100% turnover, both parameters had to be increased to their maximum coded levels.
The experimental design reconstructs the conformational dynamics of the transporter by comparing distance distributions obtained under turnover conditions with those measured in trapped catalytic intermediates.
CSF turnover was calculated by dividing the mean CSF production (μl/min) by the mean CSF volume (μl) in each age group and multiplying by 1440 min/day, thus obtaining a turnover value in day-1.
Performing adsorption tests at optimal conditions set as 0.5 g L−1 of adsorbent, pH 10 and 308 K make admit to obtain high adsorption turnover (123.45 and 140.28 mg g−1 for MgO nanoparticles and ZnO MgO nanocomposites, respectively).
To obtain k, the turnover rate, the experimental data were fitted to the equations Et = Ep 1-e−kt) or Ep 1-e−ktkt.
To obtain kcat or turnover numbers (TN), the change in absorbance was converted into NADH consumed using an extinction coefficient of 6.22 cm–1 mM–1 for NADH (ε340) or 11.9 mM–1 cm–1 for tNADH (ε398) and divided by NDH-2 concentration.
The coordination sphere can be adapted by introduction of mono- or bidentate phosphorus-containing ligands, providing efficient tools to obtain with high turnovers, regio- and even enantioselectivities the incorporation of a COOR function onto a carbon atom.
The resulting velocity was plotted as a function of metal or dNTP concentration and then fit to a hyperbolic equation, correcting for enzyme concentration, to obtain estimates for the turnover number (kcat) and Michaelis constant (KM,metal or KM,dNTP).
