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We fused two different bidirectional degrons (cODC1/2-TDegF) between the green fluorescent protein (GFP) and the red fluorescent protein mKate (RFP) to obtain tester proteins.
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As previously described [ 70], a fully penetrant and genome-integrated GFP marker with a myo-3 promoter was introgressed in the A6140 population to obtain a tester population with standing genetic diversity (A6140GFP).
A higher consistency of measurements was obtained within-testers as compared to between testers, and sensitivity analysis demonstrated a negligible influence of palpation digitization errors on the innominate angle measurements.
Conclusion: By using an HHD and a standardized measurement protocol, a novice tester can obtain reliable lower-extremity strength values in community-dwelling elderly fallers.
Note that we use (G T) h v as fixed effect, which is necessary to obtain the genotype by tester means.
To obtain the "driver" and "tester" cDNA populations, 0.6 μg of mRNA purified at 24 h, 36 h and 48 h periods were pooled for each set of conditions (FSCW and glucose).
We used the noise testers to obtain the actual noise level in the railway passenger coaches.
We used the temperature testers to obtain the average temperature in the passenger coaches, and the test data were used to calculate the air pressure change rate.
On all sampling days after day 0, samples were obtained only from tester animals.
The surface hardness is obtained using hardness tester, phase composition is evaluated by X-ray diffraction, surface features are observed using scanning electron microscope (SEM) along with energy-dispersive X-ray spectroscopy (EDS) and wear rate is assessed using the ball-on-disc tester.
Although the wear loss of materials evaluated on the artificial joint simulator under ISO standard is found to conform to that in vivo, there is a significant difference in the morphology of the wear debris obtained from the tester and human body.
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