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To obtain synchronized animals, gravid hermaphrodites were bleached.
To obtain synchronized tumors, 150 000 R2cellsls were injected subcutaneously into the right flank of each mouse.
To obtain synchronized early and late L1, viviparous adults were maintained on young plants for 6 hours.
To obtain synchronized plants at different developmental stages, plants were harvested using morphological markers for 7 16 day old plants.
On the day prior to setting up RNAi by feeding in liquid bleach gravid adults and hatch embryos overnight in M9 buffer to obtain synchronized L1 population.
Worms were allowed to lay eggs on NGM agar plates and grown at 15°C for 4-5 days to obtain synchronized populations.
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The combination of a capillary based microelectrochemical flow cell system and downstream UV vis analytics allows obtaining synchronized electrochemical and spectroscopic data in a fully automated mode.
The ease of obtaining synchronized cell populations of the Gram-negative bacterium Caulobacter crescentus through a physical method [ 3] has made this organism a prominent bacterial model for analyzing the cell cycle [ 4].
Synchronous animal populations were generated by hypochlorite treatment of gravid adults to obtain tightly synchronized embryos, which were allowed to develop into adulthood.
An algorithm to obtain Monte Carlo synchronized permutations is introduced and a comparative simulation study with the parametric ANOVA is reported.
To obtain highly synchronized larvae without bleaching, adults of the indicated genotypes were transferred onto seeded NGM plates and allowed to lay eggs for 1 hour.
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