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We harvested pancreata from liraglutide and vehicle-treated Zucker Diabetic Fatty (ZDF) rats to examine islet structure and function and obtain isolated islets.
Unfortunately, it is very difficult to obtain sufficient islets from the fibrotic human pancreas, and it appears that isolated islets are highly susceptible to rejection.
Screening for risk of type 1 diabetes uses "biochemical" autoantibody assays for specific islet autoantigens (1).
The leading hypothesis is that B* 39 alleles present specific islet peptides to CD8 T lymphocytes.
To obtain single cells, islets were dissociated in Ca2+-free buffer.
Thus, LCM represents an ideal tool to obtain native islet samples without inducing the stress-response of islet isolation.
Left video represents the recorded images; right video demonstrates the islet tracing performed by CGE plugin for ImageJ in order to obtain single islet profiles.
Prevalence of amyloid-laden islets was obtained among 100 islets and expressed as a percentage in each case.
Protein obtained from islets (50 g; ∼100 islets) were used for each experiment.
No increase in insulin content was obtained in islets from E2-treated ERαKO mice (Fig. S7).
