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Transformants were selected on medium lacking leucine at 32 °C to obtain single colonies.
Before permeabilization, cells were treated for 5 min with trypsin to obtain single cells.
These repeated measures were averaged to obtain single T1 and T2 values for each in vitro phantom.
A stereoselective synthesis was projected to obtain single enantiomer of the latter compound.
Then, the culture was subjected to separation using the LB agar plate to obtain single clones.
It is possible to obtain single crystals of sapphire and ruby on its basis.
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A new methodology to obtain single-ion-conducting polymer electrolytes is proposed.
Briefly, NP tissues were minced, enzymatically digested in 0.2% type II collagenase and 0.25% trypsin for 3 h, and filtered to obtain single-cell suspensions.
For splenic cell profiling assessment, spleens were harvested at the indicated days after infection, homogenized, and passed through a 70 μm strainer to obtain single-cell suspensions.
To obtain single-cell suspensions, lymphocytes were isolated from the thymus, spleen, inguinal lymph nodes, lungs, and livers as described before35.
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