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Following euthanasia, the mandibles and tibiae were resected, fixed, decalcified and processed histologically to obtain sections for H&E and tartrate-resistant acid phosphatase (TRAP) staining.
To obtain sections from isolated islets, islets were washed with PBS, fixed in Bouin's solution for 15 min. and resuspended in 2% melted agarose in PBS, followed by short centrifugation and paraffin embedding.
Calvariae were bisected perpendicular to the sagittal suture through the central portion of the parietal bones, parallel to lamboidal and coronal sutures, and embedded in paraffin to obtain sections of a standard area according to the method described by Zhao et al. [71].
Mammary glands were cut to obtain sections from the nipple through the fat pad toward the abdominal muscles.
The inflorescences were dissected out and cut to obtain sections of rachis tissue measuring 1 cm in length.
Macrodissection of FFPE sections to be analyzed by the HER1 VeraTag™ assay was performed to obtain sections with >70% tumor.
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Previous mayors tackled the problem with the assistance of federal programs, helping families in shelters obtain Section 8 rent vouchers and federal public-housing apartments managed by the New York City Housing Authority.
The obtained sections were observed under a Morgagni-268 electron microscope under standard operating conditions.
The obtained sections were analyzed under fluorescent (Olympus IX-70) and confocal microscopies (LSM 510 Meta Zeiss, Oberkochen, Germany).
The obtained sections were plunged within a few seconds into liquid nitrogen until all sections were collected.
As controls, we also obtained sections of non-tumor tissue from the region surrounding TNBC and LUM-B tumors.
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