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After 21 days, colonies were stained with crystal violet (Sigma) and scanned to obtain representative images.
Fluorescent images gained from LAMP2 immunostaining did not generate puncta that could be rendered to generate spherical images, instead LAMP2 staining was treated as a "surface" (which does not restrict the rendering shape to spheres) to obtain representative images.
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From the overall morphology and these measurements, we concluded that we had purified minichromosomes and obtained representative TEM images from both G1 and M phase cells.
Moreover, a montage tool allowed us to obtain final representative images of large microscopic fields, which on analysis by the Gold Finder module provided information on the distribution and localization of antigens comparable to that provided by the wide-field light microscope images.
We randomly obtained four representative images per well under the microscope with a 10 × objective, and counted all of the cells in those images for one experiment.
To obtain higher magnification representative images for each of the transfection combinations, transfected cells were fixed with 4% PFA in PBS PANN) for 10 min at RT.
Representative images obtained with this automated image analysis system are shown in Figure 1A in which each cellis outlined in red and nuclei of each live cell overlaid in blue.
Representative images obtained by brightfield microscopy showing staining for phosphates (von Kossa) from day 21 is shown in Fig 5A and for calcium deposition (Alizarin red) is shown in Fig 5B.
Representative images obtained at each time point from mice in each treatment group and from non-tumoured control mice are shown in Figure 5.
Representative images obtained at 0 hours or 12 hours after scratching showed cell migration into the scratch "wound" and a reduction of the width of the denuded cell monolayer (supplementary material Fig. S2A).
The representative images were obtained from six independent experiments.
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