Sentence examples for obtain regulated from inspiring English sources

To obtain regulated p27 expression, in the first method target cells were co-transduced with two viruses: the AdTet-ON (Clontech), that provides the constitutive expression of the reverse tetracycline-controlled transactivator (rtTA), and the AdTRE that encodes for the p27WT or mutant proteins.

Generally, even though binary systems have the disadvantage that two vectors must enter the same cell to obtain regulated expression, this design is advantageous for some applications, namely when delivering large transgenes.

The advantages of such a setting are: (i) only one virus must enter the target cell to obtain regulated expression of the transgene, (ii) exact virus titration is easier and (iii) lower virus doses are required for comparable transgene expression.

Because the purpose of combination treatment with metformin and oral contraceptives in women with PCOS and EC is to preserve their uterus for future pregnancy [25], it is best to help patients obtain regulated ovulation and subsequent normal uterine pregnancy as early as possible after co-treatment.

An added advantage of this system is that, contrary to other modulatable Cre systems, it offers the possibility of obtaining regulated recombination in a combinatorial manner, i.e. induce recombination at any desired time-point specifically in cells characterized by the simultaneous expression of two different promoters.

Furthermore we created an expression profiling database using Illumina sequencing in order to obtain genes regulated by JA.

Earlier, Baron et al. (1995) constructed tetracycline-inducible bidirectional promoters by duplicating a minimal promoter on both sides of a series of Tet operator repeats to obtain exogenously regulated expression of two transgenes in a correlated, dose-dependent manner [ 23].

6 A strict fold-change or P value cutoff is needed to obtain the regulated gene list, but the choice of the cutoff is often arbitrary and can have a significant influence on the test outcome and, subsequently, the interpretation of an experiment.

This result suggests that pair-wise gene expression changes obtained from copy number variation in the RH panel are similar to those obtained from regulated gene expression in multiple tissues of a mammalian organism.

We propose that specific molecular networks are established early in T cell activation and then additional and critical gene components of these pathways are added as a function of time to obtain and/or regulate the impact of these networks on the final result.

Electrochemical tests show that different electrochemical performances can be obtained by regulating the contents of each phase (LiVP2O7, Li3V2(Por)3, or LiVPO4F).