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To obtain quantification data from the assay, we randomly chose 3 fields to count the total cells and the BrdU positive cells, and the data were graphed as a ratio of BrdU positive cells to total cells.
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All obtained quantification results were manually screened with the spectral data.
Furthermore, reducing analysis time is often more important than obtaining precise absolute quantification data.
In order to show that mRNA levels obtained by NanoString analysis of FFPE tissues were accurate, we compared quantification data obtained using RNA isolated from paired fresh-frozen and FFPE oral cancer samples.
We also aimed to compare whether quantification data obtained by NanoString achieved a higher correlation than data obtained by SYBR Green I fluorescent dye-based RQ-PCR, using the same paired fresh-frozen and FFPE samples.
Since the initial analyses were based upon relative quantification data obtained from the non-targeted biochemical profiling technology, a targeted assay was developed to provide absolute quantitative results.
However, a major current challenge in the cell migration field lies in the development of suitable software for the annotation, management and sharing of the quantification data obtained from such imaging-based experiments.
Detection and quantification data were obtained within 48 h with the new technique, compared to 5 6 days for the classical plate culture method, while also providing greater specificity and sensitivity.
Relative quantification data were obtained with ABI Prism 7000 SDS software (Applied Biosystems).
For quantification, data were obtained from at least three independent experiments and the number of cells used for quantification is indicated in the figures.
BioMy research interests include uncertainty quantification, data assimilation.
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