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We obtained reference values for pooled plasma samples from repeated analyses to obtain quality control charts.
It was then spiked with different amounts (ranging from 20 to 2000 ng/g fresh tissue) of MDA, ACR, and HNE to obtain quality control (QC) samples for quantification according to the AIDA protocol.
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The gene expression data from all the samples were obtained, quality control steps were performed and the data was analyzed using GeneSpring GX 10.0.2 (Agilent Technologies).
The inter-assay accuracy and precision were calculated from results obtained from quality control samples (N = 6) analysed at four concentrations (0.5, 1.1, 15 and 40 ng/mL of diclofenac in EDTA plasma representing LLOQ, QCL, QCM and QCH respectively) on three separate occasions, see Table 2.
Clean reads from four samples were obtained after quality control.
Sequence reads obtained after quality control with filtering were analyzed using CLC Genomics Workbench 4 (CLC bio, Cambridge, MD).
To perform HC, a distance matrix that measures pair-wise distances between drugs was obtained after quality control.
External DENV control panels were obtained from Quality Control for Molecular Diagnostics (QCMD) in the years of 2011, 2012, 2013, and 2014.
In the future, the above phytoconstituents could be used as a major tool for obtaining a quality control profile for a drug.
In our study, the coefficient of variation of concentrations obtained in quality control samples varied between 7% and 34% [see Supplemental Material, Table 1 (http://dx.doi.org/10.1289/ehp.1103552)].
The extract concentration in the tablet was determined by standard mathematical analysis, i.e. tablet containing 500 mg of extract standardized to 50percentthatat will be 250 mg of extract.> -wrap-foot> The specification mentioned in the table obtained from quality control lab of Sree Kundeswari Aushadhalya Ltd.-Ctg.
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