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Cytokine levels were analyzed in two ways, either using raw cytokine levels when comparing cytokines between two groups of children or using net cytokine levels (substracted from the negative control) to obtain percentage of responders/non-responders (above or below zero for substracted values).
Residue obtained was weighed to obtain percentage yield.
Systematic knockout was performed to obtain percentage disruption by each gene.
The difference of the two was then divided by 30 before being multiplied by 100 to obtain percentage adherence.
The solvent- containing extracts were evaporated to dryness on a rotary evaporator under reduced pressure to obtain percentage yield of 23.8% w/w and 17% w/w respectively.
For each microPET/CT scan, 4.0 mm diameter spherical regions of interest (ROIs) were drawn over both the tumor and the contralateral muscle on decay-corrected images using Inveon Research Workplace to obtain percentage injected dose per gram (%ID/g) and standardized uptake values (SUV).
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The neural network algorithm (Softsec software; Softwood Inc., PA) was used to obtain percentages of different types of secondary structures of the analyzed peptides [21]; [21].
We used the procedure MEANS of SAS Statistical Analysis System SASAS, Cary, North Carolina, USA) to obtain percentages, medians and interquartile ranges.
Normalized values are then multiplied by 100 to obtain percentages, i.e. final scores range between 0 and 100.
Crosstabs were then calculated to obtain percentages of agreement across low, medium and high CBCL and TRF scores.
Statistical analysis was performed to obtain percentages of all of the responses and chi squared tests were undertaken to investigate for significant statistical relationships between responses and demographic variables.
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