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De-regressed breeding values for sires within the discovery set were utilized to obtain marker effects.
To obtain marker sets with various SNP densities we sequentially removed markers from the current map using every second, fourth, 10th, 50th, 100th and 200th marker (Table 2).
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To increase the public acceptance of such transgenic plants we developed a transformation procedure that uses the chemical inducible Cre-lox system to obtain marker-free transgenic Jatropha.
The first transformation using the inducible Cre/Lox system to obtain marker-free transgenics was reported to improve agronomical traits and seed quality [ 25].
Microarray analyses have been used to link changes at the level of gene expression to different kidney diseases to obtain markers for diagnosis and prognosis.
Thus, enzymes with high GC content recognition sites should be used if the interest is to obtain markers within or close to gene sequences.
Combining linkage groups in this way and using MapDisto to order the markers, we obtained marker orders that were largely consistent with the genome sequence.
A 'flipsn' analysis was then used to verify that the obtained marker order was the most likely order.
These data thus indicate that ablation is far more effective in obtaining marker antigen-loaded DC in the draining LN as compared to conventional DC vaccination.
The obtained marker map is shown in Fig. 5B with additional details provided in additional file 2. It comprises a total of 101 markers with an average distance between adjacent markers of 1.7 cM.
The MSSC-MSF uses multispectral-spatial classification for marker selection, and MSF is built on the obtained markers.
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