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We harvested pancreata from liraglutide and vehicle-treated Zucker Diabetic Fatty (ZDF) rats to examine islet structure and function and obtain isolated islets.
In general, a consistent post-treatment procedure and especially homogeneous cell dilutions needs to be ensured to obtain isolated colonies in high reliability.
Amplified phages were screened in additional rounds and then plated to obtain isolated clones.
After washing in PBS 1X solution, the muscle was teased apart in PBS to obtain isolated fibers.
To obtain isolated colonies, dilute conidial suspensions were inoculated onto either iron-limited or iron-supplemented Neiland's agar.
Each type of microorganism (from the stock culture) was streaked onto a noninhibitory NA plate to obtain isolated colonies.
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Samples were obtained, isolated, seeded, stimulated and incubated with cytotoxic drugs and BA as described.
By using that approach, it was possible to obtain isolates with improved ability to colonize plant roots without engineering their genomes.
One half was inoculated into BSK-II media to obtain isolates.
Describe the method used to obtain isolates indicating: i. Incubation period and temperature.
We were not able to obtain isolates of all published ITS sequence variants.
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