In contrast for endophilin K8 which, practically, lacks the hydrophobic insertions, we obtained generation of the tubular phase in the absence of the vesicular one (not shown).
We selected the four lines displaying this phenotype to obtain T2 generation plants.
In this work, the LNG vessel loading process is dynamically simulated to obtain JBOG generation profiles.
The male sterile plants with homozygous Pi2 were selected in BC1F2, BC2F2 and BC3F2 populations and ratooned under low temperature to obtain next generation seeds.
Females F0 were fed several times on guinea pigs to obtain F1 generation eggs.
T1 seeds were collected at maturity and this process was repeated to obtain T2 generation seeds.
F2 founders were then in-crossed to obtain F3 generation homozygote mutants, which were used for phenotypic and genomic analysis.
We let F1 hybrids self-pollinate to obtain a generation of autogamous hybrid progeny (hereafter called S1).
Six of the 13 founders were backcrossed to the wild-type Ntac SD strain to obtain N1 generation: 311, 33, 34, 36, 38 and 40.
After 48 h, individual spheres were formed, dissociated with accumax (Millipore, Temecula, CA, USA) and 20000 single cells/mL were used to obtain second generation of spheroids.
