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No information was available about anesthetics used to obtain frozen samples from CHTN.
Paraformaldehyde-fixed brains from P14 and P20 mice were used to obtain frozen sections that were immunostained with a free-floating section-staining protocol.
Even when special handling methods are used to obtain frozen tissue, there may be RNA degradation; for example autopsy samples where degradation occurs both pre-mortem and during the interval between death and cryopreservation.
In order to obtain frozen ultrathin sections, the segmented blocks of gelatin-embedded cells were mounted on ultracyotome stubs, and frozen in liquid nitrogen.
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For fear conditioning experiments, mice were habituated in individual conditioning chambers to obtain freezing baseline on the first day.
Such exotic meats are usually obtained frozen and reach the table desiccated and denatured.
Plasma, PAXgene RNA, and DNA samples were obtained, frozen, and stored for future biomarker discovery studies.
We obtained frozen and paraffin-embedded (formalin fixed) MCC and SCLC biopsy and resection samples from the archives of the Pathology department of the Radboud University Nijmegen Medical Centre.
Bovine brain microvascular endothelial (BBME) cells were obtained frozen from Cell Applications Inc. (San Diego, CA) and were grown in 75 cellculturelture flasks coated with collagen.
Vero cells were obtained frozen from ECAGC, Salisbury, UK.
They were obtained frozen in liquid nitrogen (−180 °C) from the American Type Culture Collection Organization (USA).
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