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Interestingly, 20 vectors, out of 29, whose PCR products could not be detected by E-Gel could still be transformed and obtained as entry clones successfully.
Indeed, removing the gold using a resin-bound scavenger Reaxa QuadraPure™ MPA[ 10f] prior to purification by column chromatography solves the oxidation problem and allows the allylic thioether 3 j to be obtained successfully in 56 % yield (entry 11).
At each focal position a ptychographic phase image was obtained and successfully refocused post-acquisition.
Isokinetic measurements were obtained after successfully finishing the FFT.
Full informed consent was obtained before entry into the study.
Importantly, the pure Ni-SSs were obtained successfully.
In every case, transformed colonies were obtained successfully.
The obtained expressions were successfully validated with Monte Carlo simulations.
The sections with immunohistochemical staining were obtained successfully.
25 ~ 50 oocytes were obtained from each successfully supervulated mice.
Informed consent was obtained upon entry into the cohort.
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