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To obtain differentiation of lung cancer sphere-forming cells, stem cell medium was replaced for 3 days with Bronchial Epithelial Cell Growth Medium (Cambrex, East Rutherford, NJ, USA) in tissue culture-treated flasks, to allow cell attachment and differentiation.
In order to obtain differentiation of lung cancer sphere-forming cells, stem cell medium was replaced with bronchial epithelial cell growth medium (BEGM, Lonza, East Rutherford, NJ, USA) in tissue culture-treated flasks to allow cell attachment and differentiation.
To obtain differentiation, H9C2 cells (provided by Dr. Tomas Simunek, Department of Biochemical Sciences, Charles University in Prague, Czech Republic) were continuously cultured for 14 days: 3 days in 10% FCS medium and the following 11 days in 1% FCS medium.
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We obtain the differentiation towards tubular cells of ARPCs cultured in basal medium conditions, the tubular commitment thus being specifically induced by micropatterned substrates.
We present computational results showing that (i) good quality solutions can be computed and (ii) proper load balancing can efficiently obtain service differentiation.
A perusal of the literature on hNSCs, readily underlines how one of the major hurdles in this area has been to obtain the differentiation of hNSCs into a meaningful and useful amount of oligodendrocytes [31] [34].
To obtain further differentiation, we performed neutralization assays against MERS-CoV and bovine CoV (7 ).
We therefore sought a procedure by which we could obtain reproducible differentiation in liquid culture.
We used the murine MSC C3H10T1/2 to obtain chondrogenic differentiation in micropellets.
Preventing the licensing of sites of noncanonical replication might be a more fruitful approach to obtain sustained differentiation of cancer cells.
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