Sentence examples for obtain conditioned from inspiring English sources

To obtain conditioned media from homotypic cultures or cocultures, 1.5×106 cells of each type were plated in a 150 cm2 dish and incubated in a 1∶1 mixture of CA1a- and fibroblast medium ("coculture medium").

The aneurysmal wall was fixed in paraformaldehyde (3.7%) for immunohistochemical analysis or incubated for 16 hours in RPMI-1640 at 37°C (Invitrogen, Cergy-Pontoise, France) (6 mL/g of wet tissue), in order to obtain conditioned medium.

In order to obtain conditioned medium from senescent or apoptotic H9c2, we treated the cells for 3 hours with 0.1 or 1 µM doxorubicin, respectively, in growth factor-free DMEM plus 0.1% BSA, then the H9c2 were grown in fresh medium (growth factor-free DMEM plus 0.1% BSA) for 24 hours [9].

Cells were cultured for 4 days to expand the culture and to further obtain conditioned medium.

To obtain conditioned medium cells were grown to confluence during 48hrs before collecting the supernatant.

To obtain conditioned medium, fibroblasts and astrocytes were grown for 24 h in Roswell Park Memorial Insitute (RPMI) without serum.

But when they obtain conditions favorable to their strengths, they can be mighty soldiers.

Online readers are also able to obtain condition reports for every featured artwork, as well as prices for that work or a comparable one at auction going back as far as 1985.

After substituting (4.12) into (4.11) we can obtain condition (4.1).

Applying a similar argument as in the proof of Theorem 2.1, one can obtain condition (ii).

We obtain conditions of oscillation and nonoscillation for Runge-Kutta methods.