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Scheme 2 Synthetic pathways used to obtain compounds 2-16.
Scheme 2 outlines the synthetic pathway used to obtain compounds 3 16.
Apparently, water is necessary for such reactions because attempts to obtain compounds 8a-l under aprotic conditions were unsuccessful.
The eluent used were n-hexane, EtOAc, DCM and MeOH in gradient manner to obtain compounds 1 3.
A convenient synthetic route via fulleropyrrolidine 2 was employed to obtain compounds labeling agents 5 and 9.
The reaction of the isocyanate functionality with morpholine was used to obtain compounds for determination of the composition by NMR-spectroscopy.
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Animals obtain compound nitrogen by eating plants or other animals.
C1A (56.0 mg), and further purified by a silica gel column (CHCl3) to obtain compound 6 (9.0 mg).
Koshimura and coworkers obtain compound 6, by hydroxylation of androstenedione with Gelasinospora retispora[22].
Fr.IV.4 (0.5 g) was chromatographed repeatedly over HPLC (SB C-18 column, CH3CN-H2O, 7:3) to obtain compound 7 (5 mg) successively.
After cooling, the resulting solid was filtered, dried and recrystallized from ethanol to obtain compound 3 (1.77 g, 75%).
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