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The gelatin silica sol treatment step is particularly slow, mischievous, and requires comprehensive downstream processing to obtain clarified juice.
To obtain clarified lysate, each pellet was resuspended in 8 ml of 100 mM EPPS (pH 8.2) and lysed by sonication, while being kept on ice.
To obtain clarified lysate, each pellet was resuspended in 8 ml of 100 mM [4- 2-hydroxyethyl -1-piperazinepropanesulfonic acid] (EPPS), pH 8.2 and lysed by sonication, while being kept on ice.
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The results obtained clarified that the proposed SIA-CL method exhibits linear concentration ranges at 1.0×10−9-1.0×10−2 moL−1−1.
The data obtained clarified the formation of an interfacial chemical bond between ZnO and SiO2 as affirmed by FT-IR and XRD analysis.
This cell suspension was subjected to two brief rounds of sonication, followed by a clarifying spin to obtain a clarified cell lysate.
The homogenate was centrifuged at 13,000 g for 20 min to obtain a clarified supernatant.
To obtain a clarified supernatant for recombinant protein purification, a primary centrifugation step was performed at 3,900 × g.
The homogenate was centrifuged at 13,000 g for 20 min at 4°C to obtain a clarified supernatant.
Nitrate was extracted from the tissues by homogenizing the samples previously boiled in 4 volumes of distilled water for 15 min. The homogenate was centrifuged at 12,000 g for 20 min to obtain a clarified supernatant.
The cells were then lysed in polysome lysis buffer (10 mM Tris-Cl, pH 7.4, 5 mM MgCl2, 100 mM KCl, 1% (vol/vol) Triton X-100, 0.5% (wt/vol) deoxycholate, 1000 U/ml RNasin, 2 mM DTT and 100 µg/ml Cycloheximide in DEPC-treated water), incubated on ice for 10 min and centrifuged at 16,000× g at 4°C to obtain the clarified post-nuclear lysate.
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