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More importantly, users can compare different ChIP-seq datasets for the same protein and obtain ChIP peak information on a specific locus.
GeneChip Operating Software (Affymetrix GCOS v.1.4) was used to obtain chip images, with quality control performed using the AffyQCReport software (Bioconductor, Berkeley, CA, USA).
The tag mapping coordinates were extended 200 bp to the 3′ direction to obtain ChIP DNA fragment locations on the mm8 genome.
The main advantages of this basic ChIP assay over previously published conventional protocols [[ 20, 31, 37], for example] is the combination of speed (only about 2 hours to obtain ChIP samples, via the elimination of some common steps) and sensitivity (known targets could be detected with only about 20,000 cell equivalents were used per PCR reaction).
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High strength alloy steel AerMet100 was employed in orthogonal cutting experiments to obtain chips at different serration stages and corresponding machined surfaces.
Both elmwood and pinewood were debarked, cut into smaller pieces, and milled to obtain chips of less than 2 cm.
The obtained chip characteristics in combination with the sensitivity corrected probe-intensity values provide expression estimates scaled in natural units which are given by the binding constants of the particular hybridization.
In this paper we report an investigation on the processing of magnesium samples by filing and the hydrogen storage properties of the obtained chips.
We obtained ChIP-seq peaks from the C. elegans modENCODE consortium (Araya et al., 2014).
Obtaining ChIP-seq results from primary breast tumors was the next step that everyone was eagerly awaiting.
We obtained ChIP-seq data for Stat3 and a GFP control from a previous study (Chen et al, 2008).
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