Sentence examples for obtain assembly from inspiring English sources

In addition, to obtain assembly statistics profile about reads that could be mapped back to the assembled unigenes, TopHat (version 2.0.8) (released 2013-02-26, http://tophat.edu/.umd.edu/) [ 44] with the parameter "mate-inner-dist = 250", was used to align short reads to the constructed transcripts by de novo assembling.

Finally, the genome-matched novel transcripts (after removing chimeras) and nongenome-matched novel transcripts (after blasting to nr database) were merged separately for Velvet-Oases and Trinity to obtain assembly specific novel transcripts.

We used SparseAssembler (Ye et al., 2012) to obtain assemblies from base-calls obtained by each method.

In doing so we will perform an extensive validation of obtained assemblies.

The MITOS web server (Bernt et al. 2013) was used for annotation of obtained assemblies.

We decided to follow this approach and examine whether the obtained assemblies can spontaneously reduce ionic silver to silver nanoparticles.

Subsequently, the obtained assemblies were further processed with SSPACE [ 45] to scaffold, and Gapcloser [ 43] to close the gaps in a final step.

In an effort to impart adhesiveness to the obtained assemblies, we incorporated lysine (Lys) into the Fmoc DOPA DOPA building block.

Of the obtained assemblies, contigs comprising sequences with different lengths of the same SSR motifs (different numbers of the same repeated sequence) between two lines were selected as polymorphic SSR candidates for primer design.

As a result, users may obtain multiple assembly products as matches to a query.

Various parameters, including k-mer length, insert length and expected coverage, were optimized to obtain best assembly as described previously.