Ct extension in SiCYS/Group II phytocystatins presumably did not obstruct the binding of its Nt region to papain, but improves the inhibitory activity against papain bases on the studies with CeCPI (Wang et al. 2008) and SiCYS here.
Conversely, the sensitizer (e.g., Bad) could obstruct the binding of Bax to Bcl-2.
Among the ~28 million CpG dinucleotide sites that are susceptible to methylation in the human genome, approximately 10% are in the promoter regions of genes, in which they may physically obstruct the binding of transcriptional proteins to the gene or may be indirectly regulated by the recruitment of methyl-CpG-binding domain proteins through cytosine methylation [ 14- 16].
Structural modeling has demonstrated that DMIs bind CYP51 at the sixth ligand of the heme group, and it has been suggested that the Y136F mutation confers resistance by obstructing the heme-binding site [ 21].
Mutations within this structure can obstruct the channel and affect the binding of macrolide antibiotics [ 19].
In general, HDACs catalyze the deacetylation of nucleossomes, that become highly condensed, obstructing the access of transcriptional activation factors to their binding sites and, therefore, resulting in transcriptional repression.
Indeed, recent work suggests the primary role for Cyc8 Tup1 involves obstructing the activation domains of the DNA binding proteins responsible for its targeted recruitment [23].
Thus, crystal structures of MtbHadAB bound with flavonoids illustrate that butein, fisetin, and HCC exert their inhibitory effect by obstructing the placement of the fatty acid into the substrate binding site of MtbHadAB.
(A) The binding of MCM2 and ASF1 obstructs the formation of NCP and a histone H3-H4 22 tetramer.
By obstructing the rotation of the central stalk, the V1-ATPase A3B3 complex can stop cycling between the three nucleotide-binding states thus preventing the unwanted hydrolysis of ATP.
