As a positive control for successful AQP inhibition we used mercury ions (Hg2+), which are capable of binding with a cysteine and an alanine within the AQP pore, thus obstructing water transport through the channel.
Hg2+ ions interact with a cysteine residue close to the NPA motive in the E loop and an alanine residue in the B loop of most AQPs and efficiently obstruct water transport through the pore [13].
This should be because the entrapped cell structure did not obviously obstruct the substrate transport (more detail of entrapped cell morphology presented in later section) (Siripattanakul and Khan 2010).
Based on our Chl fluorescence data RLCs and OJIP kinetics, changes of Chl fluorescence variables Fv/Fm, rETRmax, Ek, Vj and Vi (Figs. 3, 4, 5, 6) we believe that higher Se doses obstruct photosynthetic electron transport between PSII and PSI due to the over reduction of plastoquinone electron carriers.
Leading hypotheses are that EPS physically obstructs plant water transport, or that EPS cloaks the bacterium from host plant recognition and subsequent defense.
The hallmark of the FBR is the formation of a thick, avascular collagen capsule surrounding the sensor, isolating it from the tissue and obstructing mass transport of interstitial glucose to the sensor.
Alternatively, the internal diameters of these dendrites are much smaller than their axons suggesting any impairment of cytoplasmic flow – by diffusion or transport-would be preferentially obstructed in the dendrites.
She was so long her bow obstructed the slipway's railway facility, used for transporting material around the yard.
Women whose labours were considered to be obstructed during daytime would find it easier and less costly to get means of transport as opposed to the women who faced a similar condition at night.
In continental Europe the impact was less marked, because the great natural rivers already linked by artificial waterways constituted an international network providing transport economically without transshipment; the terrain was more favourable and the canals larger and less obstructed by locks.
