The VHS domain has been implicated in ubiquitin binding and in interaction with the carboxy-terminal part of RNA Pol II; it has also been suggested that the UVSS-A mutation Cys32Arg might obstruct interactions between the VHS domain and ubiquitinated proteins.
Alternatively, phosphorylated Tyr18 (pY18) and Ser13 (pS13) in FUNDC1 significantly obstruct their interaction with the hydrophobic pocket and Arg10 of LC3B, respectively.
> -wrap-foot> is is now widely accepted that druggable proteins are highly conserved because substitutions in the interface may obstruct target drug interactions (Wang, Wang, et al. 2013).
For instance, obstructing the interaction between HIF-1α and the co-activator CBP/p300 led to attenuation of HIF-induced gene expression and inhibition of tumour growth in a xenograft model [ 42].
Hence, the failure of S. aureus to induce TLR-mediated NF-κB activation in pbMEC is not due to just obstructing the interaction of relevant PAMPs with TLRs (TLR2 in particular) through FCS.
Direct measurements on biological membranes are obstructed by interactions of chemicals with proteins and other membrane components, complex membrane architecture, and variability of membrane composition.
To obstruct peptide opioid receptor interactions, the opioid peptides were labeled with TAMRA at the N terminus.
After careful analysis, it became apparent that crystallographic contacts obstructed the key interaction driving the observed SAR, namely, formation of a salt bridge between Arg82 and the anionic substituent of this class of inhibitors.
Interaction can obstruct or promote four clusters of barriers between research and policy: expectations, transfer issues, acceptance, and interpretation.
Two drawbacks of the existing studies are that (1) they require large supporting equipment like an incubator and a syringe pump; which involves more culture media volume, and that (2) the trapped cells are isolated from one another that cell interaction is obstructed or not considered.
