This poses a particular obstacle to functional studies since it is often the case that a gene, when mutated, will display no detectable phenotype [5], [8].
A major obstacle to functional investigations is the difficulty in producing structurally defined GPI moieties and GPI-anchored proteins.
In fact, in our war-wounded population, it may be the most significant obstacle to functional mobility and return to an active lifestyle or even active duty, affecting amputee and non-amputee patients alike.
Two obstacles to establishing functional roles for the many candidate sexual development genes that exist remain the time and cost of generating mice lacking the relevant gene, and the absence of any reliable in vitro assay for gene function during mouse gonadogenesis.
Major obstacles to a functional cure are intricate stability of HBV cccDNA and dysfunctional anti-HBV immune response.
There remain significant challenges to delivering new knowledge to the clinician and patient, including the bioinformatics challenges of identifying a causative mutation out of the >60,000 variations likely to be detected in any individual, and the technical obstacles to the functional analysis of iPSC-derived organoids.
Just as mass-univariate inference has not been an obstacle to discovering functional networks with functional MRI, so it would not have been a (major) obstacle to discovering such networks with lesions if lesions had the spatial properties of blood oxygen level-dependent response.
It is unusual, even in experimental animals, for interventions in chronic spinal cord injury to have a detectable benefit because of the numerous obstacles to regeneration and functional recovery at this stage (Kadoya et al., 2009).
The key obstacle to developing functional genotyping assays is that such mutations need to be genotyped regardless of their location in genomic regions.
Heterotopic ossification (HO) is perhaps the single most significant obstacle to independence, functional mobility, and return to duty for combat-injured veterans of Operation Enduring Freedom and Operation Iraqi Freedom.
