Fourteen genes were randomly selected, from significant differentially expressed DTA tags between the two libraries, to observe their expression patterns during shading treatment by qRT-PCR.
We observed their expression patterns and found them consistent with the microarray results in most of the cases.
RT-qPCR expression analyses with 10 genes identified on the Glc-library were also conducted to observe their expression profile during the growth of T. harzianum in FSCW.
CRABPs are intracellular RA binding proteins that are thought to shuttle RA to the nucleus to regulate RA-mediated transcription, which may explain why we observe their expression during limb regeneration (Noy, 2000).
For all differentially expressed miRNAs, in order to observe their expressions along with the infection process, a heat map was drawn, and clustering analysis was conducted based on similar expression patterns.
Not knowing what to expect, I observed their expressions of fear, insecurity, and uncertainty.
It is interesting to note that the professionals in the South African schools indicated that they knew when children with cerebral palsy were in pain by observing their facial expressions or body language, and said that this may have been because they had worked closely with the children from as young as 3 years of age.
Make a point of trying to read between the lines and pick up on people's true feelings by observing their facial expressions and other body language.
The model allowed for performing virtual experiments on the network and observing their influence on the expression dynamics of the genes downstream in the regulatory cascade.
However, the two earlier studies failed to detect different expression pattern of gene isoforms, whereas our study was the first to identify many isoforms of the isoflavonoids biosynthetic genes, and observed their different expression patterns among tissues.
