In addition, no difference in the expression of Lhx6 was observed in tangentially migrating interneurons or in the cortex of E18.5 wild-type and Satb1 mutant embryos.
In addition, ganglion cells are observed to migrate tangentially as much as 150 µm at this stage in the mouse retina [61], [62].
Also, the fact that the observed data fall tangentially outside the neutral distribution for both orientations suggests that demography may have a stronger contribution than selection to the observed departure.
Using immunofluorescence on E14.5 forebrain cryosections from control (dhet) embryos we observed YFP+ interneurons migrating tangentially forming the characteristic two cellular streams in the marginal zone (MZ) and the intermediate/subventricular zone (IZ/SVZ) and entering the dorsal telencephalon ([ Tanaka and Nakajima 2012; Marin 2013]; Figure 1 A, arrowheads indicate the MZ and IZ/SVZ).
By experimenting, observing, and then observing who is observing.
Curiously, we observed that many neurons stopped tangentially migration, paused, and then began radial migration.
The fact that we observe iACs and dACs migrating tangentially even after they have been separated into two distinct populations by the BC plexus is consistent with a recent study showing that multiple-EGF-like-domains 10/11 proteins facilitate mosaic formation of both INL and displaced starburst ACs despite the fact that the two mosaics are independent of each other (Kay et al., 2012).
When the cell tracker was placed in the SPV, we observed some cells invading by tangentially migration parts of the medial extended amygdala, including the medial BST and the medial amygdala (Fig. 12), as previously reported (García-Moreno et al. 2010; Bupesh et al. 2011a).
Many of these migrating neurons display nontangential orientation as opposed to the tangentially oriented migrating neurons observed at earlier stages (E47 E55).
We also observed iACs (n=2) and dACs (n=2) migrating tangentially away from their clonal column after dACs in the area have been separated from iACs by the BC axonal plexus.
In organotypic slice culture experiments, tangentially migrating cells were only observed in the SVZ/VZ in large numbers for the first time at 14 PCW, however, 70% of these expressed both DLX1/2 and ASCL1, and are thus likely to have been generated intracortically (Letinic et al. 2002).
