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Parasite viability was measured after 24, 48 and 72 h, by observing motility under an inverted microscope.
Killing effects on zoospores were assessed by observing motility.
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Grant had studied the organism and related species for some time and had published numerous papers on the subject, but he never observed motility in these cells so he might have been distressed that this young neophyte was successful in observing some phenomena he was unable to see.
The observed motility was comparable to the bidirectional movement of Rrm4-Gfp and Pab1-Gfp that are known to shuttle on Rab5a-positive endosomes (see below; Baumann et al., 2012; Baumann et al. 2014).
We began by observing dynein motility at the single-molecule level over a range of Lis1 concentrations.
Attempts were made to assess the viability of the inoculum by observing sporozoite motility after excystation of the oocysts by different methods.
The albendazole sulfoxide treatment induced a slightly reduction of the movement; however, body straight shapes and absence movement were not observed; thus, motility assay could not be properly performed (data not shown).
We could not observe any motility of Kpp6 in our study, thus this MAPK probably does not depend on EE motility to regulate effector expression.
To confirm the involvement of S100P in regulation of tamoxifen induced cell motility, we conducted functional studies of S100P by overexpressing the protein in the parental MCF-7 cells and observed increased motility in MCF-7-S100P MCF-7-S100P MCF-7-S100P
We observed a motility phenotype for two of these three genes.
However, when Darwin observed the motility in the so-called ova later discovered to be larvae of Flustra carbacea, a primitive invertebrate (belonging to Ectoprocta), commonly called "sea-mats," Grant's "response was chilly" when Darwin attempted to show Grant his discovery (p. 67), and he appropriated Darwin's observations as his own.
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