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We realigned all reads within candidate SV sites using Smith Waterman-based alignment utilities provided through the Biostrings package (Pages et al., 2013) in Bioconductor (Gentleman et al., 2004), with parameters reflecting the probabilities of observing alignment errors, explained further in Section 2.4 and Supplementary Material 'Smith Waterman alignment parameters' (Malde, 2008).
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The paradox is that, for the latter type of pinning site, one still observes alignment of Ld domains along the actin fibers, albeit weak.
Optimal catheter access to all pre-defined control points and the quality of CT segmentation and 3D CT reconstruction may constitute further reasons for observed alignment inaccuracies in the mentioned areas.
A simulation model that incorporates these ingredients is able to reproduce the experimentally observed alignment of Ld domains, even when the pinning sites themselves energetically favor the Lo phase.
Analyzing the course of the Risk Model project and as indicated in the findings, we observed alignment efforts made by both RIVM and the Inspectorate; investigators, inspectors, and managers tried to create alignment within the bounds of their reach, both according to the institutional procedures at stated moments and according to the circumstances in the project.
However, the number of substitutions was different between the expected and observed alignments; therefore, the read was classified as incorrectly mapped.
As an alternative option, we further extended the DP alignment to observe constrained alignment space by only considering gap open and gap extension events that can be accommodated by the reference MSA.
The prominence of this dimension, in both samples, reinforces how much subordinates perceive the leader's morality, observing an alignment between his/her discourse and action (Avolio and Gardner, 2005).
The likelihood of observing the alignment under the evolutionary model with tree 𝒯 and rate matrix Q is (1) where the summation is over all variables in the ancestral nodes of the tree.
By observing the alignment of all previously known and newly discovered isoenzymes (Additional file 2), existence of signal sequences also in other previously known and most of the new isoenzymes seemed very probable.
Due to the similarity in the alignment structure, it was observed that alignment of the functionalized CNFs was independent of the functional groups attached to the CNFs.
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