We did similar analyses in south Asians (figs 4 and 5), and observed weaker associations than in east Asians.
However, the sample size was small because the strength of the relationship that we observed was weaker than the relationship that we had assumed from the previous studies.
In addition, we observed significantly weaker than normal activity in Parkin mutation carriers in lateral orbitofrontal cortex (inferior frontal gyrus pars opercularis, Brodmann area 47), an area that is probably associated with stimulus evaluation and value representation.
When we only adjusted for age and dense area like Stone did, we observed a weaker effect than in our fully adjusted models; however, it is still statistically significant (OR 1.8, 95% CI 1.1 to 2.7 vs. 2.4, 95% CI 1.3 to 4.2).
Therefore, the effects observed are potentially weaker than they would have been if the comparison had been restricted to cities not implementing any new prevention strategies.
Finally, as the scales we used are not perfectly reliable, random misclassification may have caused the relationships we observed to appear weaker than they are in reality.
After IP administration, an increased expression of CXCL10 (57-fold), CXCL1 (16-fold) and CCL7 (5-fold) in the mucosa of adult mice was observed, but these upregulations were weaker than those observed in neonates (700-, 23-, 75- and 62-fold greater for CXCL10, CXCL1, CCL2 and CCL7 respectively).
In all cases, the degree of suppression observed by RNAi was significantly weaker than that observed in the suppressor lines.
This is 8-fold weaker than observed by FDS-SV, highlighting commonly observed (but protein dependent) discrepancies of affinities for surface-immobilized molecules versus solution affinities.
To further assess the reviewers' question, we used immunoprecipitation assays and observed that p85β homodimerized and interacted with PTEN but the interactions were much weaker than observed for p85α.
