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A172 cells were transfected with FANCD1/BRCA2 siRNA for 48 h, and the expression of the protein was observed using Western blot analysis (Fig. 5a).
Diffuse p-STAT3 staining was observed throughout the muscle fibres and appeared to intensify over the time course (figure 4a b), which agreed with the increased cytoplasmic p-STAT3 at 24 hours post observed using western blot analysis.
The identity of an additional band observed (using western blotting with anti-SMP30 antibody) in liver extracts of normal 7 day old rat is unclear at present, but it was absent (using western blotting with anti-HA antibody) in liver extracts of Ad-SMP30-HA injected mice suggesting the possibility that the anti-SMP30 antibody may have non-specific cross-reactivity.
The effect on the molecular level of apoptosis was observed using Western blot analysis.
Expression of AtTCTP over time was observed using Western blot analyses.
Expression levels of cell cycle-related genes were observed using western blotting analysis.
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Differences in PrPSc abundance were not observed using either western blot or 96-well immunoblot between 0 hr HY TME controls homogenized in pure H2O or DPBS (data not shown), indicating that there is no inherent difference in PrP detection ability between these homogenate solutions.
Due to problems with high background observed using alternative western blotting protocols, non-reducing anti-PrxIV blots of SP cell derived material were performed exactly as described previously (Tavender et al, 2008).
The validation of the protein abundance observed using 2D-DIGE was performed using western blotting.
Similar findings were observed using qRT-PCR and western blot analysis of 10 fresh RCC tumours.
The upregulation of PAFR in CDDP-treated ovarian cancer cells was observed using realtime PCR and Western blot.
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