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Because I had observed swelling of the 526N sample during polishing and rinsing (see experimental methods), I hypothesized that the matrix effect could be associated with hydration.
We observed swelling of GFP-positive cells, which resulted in expansion of the wing pouch region.
In a study by Pech et al., ablated tumours were resected directly after IRE and they observed swelling of cells but no actual cell death.
As in glia, at 5 dpf, we observed swelling and fragmenting of the ER, again indicative of ER stress (supplementary material Fig. S4).
The observed swelling of the PBSA gels in the β-ME solvent suggests that the 17 disulfide bonds remain intact during the fabrication of the gel.
The observed swelling effect during β-ME exposure could be explained by a combination of increased hydrogel and protein flexibility due to reduced inter- and intramolecular disulfide bonds that enable the hydrogel to absorb more water.
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Open image in new window Fig. 5 Schematic of the flow cell apparatus used to observe swelling and spalling of fines from mudstone and sandstone samples.
If we include deformation of anticlinal walls we observe swelling of the lobed regions (Author response image 8B).
We occasionally (7%; n = 50) observed swellings of the M1 axon in the procorpus.
The admission of Na+ leads to the uptake of water, producing the observed cellular swelling and vacuolization that occurs when the ER and mitochondria swell.
In the present case, we observed bilateral swelling of the parotid and submandibular glands.
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