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;observed rate of 1.0 ct/s.
The predicted and observed rate constants were in good agreement.
k, Top, observed rate constant (0.52 min−1) for HHARI-lysine mediated single-turnover E2 Ub discharge.
j, Top, observed rate constant for UBE3C-lysine mediated single-turnover E2 Ub discharge was too slow to measure.
The actually observed rate was about 50percentt higher than on a similar anonymous survey given to the MIT students.
i, Top, observed rate constant (0.024 min−1) for MYCBP2(cat -threonine-mediated single-turnover E2–Ub discat -threonine-mediatedn-gel fluorescat -threonine-mediatedubiquitin.
Observed rate constant of the association reaction.
Second, the active fraction cleaved with an observed rate constant that was higher than that of the ribozyme without the upstream sequences.
Open image in new window Figure 6 The observed rate constant K obs.
dUMP was consumed with a single exponential giving an observed rate constant of 0.7 s–1.
**Computed as True rate = Observed rate × (PPV + NPV-1 -NPV NPV-1 -NPV
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