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However, rare events in which phage psbA did not cluster with their hosts and did not have psbA isoforms consistent with that of their hosts were also observed (PCR based observations reported by [ 16]).
We observed PCR products under these conditions only when first strand synthesis was conducted with primers complementary to the predicted mRNA.
In cases where outlying values were observed, PCR Array experiments were repeated to confirm the results.
They observed PCR amplification of CyD3.2 and CycD3.3, while no amplification of cycA.1, CycB1.1 and CycD3.1 was observed.
When no PCR product bands were observed, PCR experiments were repeated at lower annealing temperatures (51 - 49°C).
In our study, we used the NucliSens® easyMAG® for extraction because we observed PCR inhibition when the manufacturer's recommended method was followed.
Similar(46)
However, we did not observe PCR amplification of the nuclear DNA-encoded CDH1, CDKN2A, or LINE-1 in the extracted platelet mtDNA, demonstrating absence of nuclear DNA in these samples.
In the one sample that was PCR and sequencing positive, but negative in microscopy, a weak band was observed in PCR that yielded a sequence.
Protein validation of the guaA essentiality observed by PCR was approached by shotgun proteomic analysis.
Positive amplification of 896 bp fragments specific for aacC2 genes were observed by PCR designated for the detection of the aminoglycoside resistance genes.
No amplification was observed in PCR control reactions containing only water as the template.
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