Lastly, given observed lapses in process care measures, such as A1c testing for patients with both RA and diabetes, additional research should investigate optimal co-management or shared disease-management and prevention strategies among specialty and primary care providers.
We observed certain lapses in the immediate newborn care skills assessments that could be critical for the survival of a newborn.
The normal chlamydial developmental cycle from infection to cell lysis was observed by time lapse interference contrast microscopy (see Figure 2A and supporting video S1).
The wound healings were observed by time lapse microscopy, and images were taken every 10' for 12 h.
In addition, we observed in time lapse movies that Vps32-mRFP decorates tubular structures that transiently connect cytosolic aggregates with vacuoles and undergo extension and subsequent shortening (Fig. 5C and Supplementary movie 2).
It can be expected that such studies may yield similarities to what we and others have observed in time lapse studies of CUL3 knockdown cells throughout mitosis (Fig. 1C) (Sumara et al., 2007).
In more recent work, mDia1-3 have been shown to induce filopodia in neuronal cells when overexpressed on their own., However, only mDia1 was seen within filopodia, as observed by time lapse imaging of live cells., The lack of mDia2 and mDia3 in neuronal filopodia implies that these Drfs might be involved in the initiation of filopodium formation but not the elongation of the structures.
The dynamics of globular and filamentous actin equilibrium have been analyzed using the fluorescence recovery after photobleaching (FRAP) technique: a distinct region of MCF7 mammary cancer cells transfected with green fluorescent protein (GFP -tagged β-actin has been bleached by laser (488 nm) and the actin filament dynamics were observed by time lapse microscopy.
(A, B ) Corynebacterium glutamicum L-form strain grown in nutrient broth (NB /L-form supportiNB /L-form(Msupporting-cycloserine (A ), and Escherichia coli L-formediumin RMSM5 (ΔmurA) growithn nutrient agar/MSM (B ), were observed by time lapse phase contrast microscopy.
To represent cellular dynamics I strive for the look and feel of movements observed under time-lapse microscopy.
