We observed increased levels of HIF-1α in hypoxia compared to normoxia for all cell lines tested, which is consistent with previous studies53,54.
We also observed increased levels of NEU2 and NEU3 in mouse and pulmonary fibrosis, and observed that TGF-β1 can increase NEU2 and NEU3 in PBMC, and NEU3 in epithelial cells and fibroblasts.
In infected TCM cells, we observed increased levels of p-ERK shortly after stimulation and elevated levels of p-p38, p-JNK, and p-IκBα at later time points across multiple donors.
To illustrate the utility of this labeling, we tagged the NF-kappaB p50 transcription factor with benzophenone, cross-linked with UV light, and observed increased levels of p50 homodimerization in the presence of DNA and the binding protein myotrophin.
In bleomycin-induced fibrotic lesions in mouse lungs, we observed increased levels of ST3GAL2 and ST6GAL2, and no change in levels of ST8SIA1 (Supplementary Fig. S1), suggesting that the reduced sialylation observed in Fig. 1a e is not due to reduced levels of these 3 sialyltransferases.
Indeed, we observed increased levels of Clocel_0591 (transaldolase), which is related to the pentose phosphate pathway, in xylan medium.
We also observed increased levels of this protein in the lungs of mechanically ventilated mice, particularly after concurrent exposure to high FiO2.
Similarly annotated genes are part of mannan metabolic pathways in other bacteria (Senoura et al. 2011), and we observed increased levels of proteins in this pathway (Clocel_3198, 3205) in the presence of galactomannan.
Consistent with increase VEGF mRNA expression, we also observed increased levels of secreted VEGF protein (Figure 4B).
We have observed increased levels of both 2-hours glucose and HOMA values associated with the UCSNP44 TT genotype.
We also analyzed the cell lysates for the invariant surface glycoproteins ISG65 and ISG75, and observed increased levels of both after four hours MG-132 exposure.
