Exact(3)
To determine if any of the observed functional consequences of altered ATP supply capacity are also associated with matrix adhesion and extension as seen in spreading astrocytes, we performed spreading assays with the MEF lines (Figure S3).
We therefore sought an alternative explanation for the observed functional consequences of the TRPC3 Mwk mutation.
Although the JAK2V617F mutation has been detected in HSC-enriched populations of human MPN patients (Jamieson et al., 2006), there were no observed functional consequences in LSK cells, such as differences in cell cycling (as measured by BrdU and 7AAD incorporation) or JAK-STAT signalling (as measured by flow cytometric analysis of phosphorylated STAT5).
Similar(57)
Is inhibition indeed as weak as shown in the study or is the applied protocol insufficient to observe functional consequences?
Nor have we established whether the promoter CpG methylation events we observed have functional consequences for transcription.
Although NKG2D ligands on Dex were different between NV and melanoma patients, we could not observe any functional consequences regarding NKG2D-dependent NK cell activation in vitro (data not shown).
Since γc cytokines trigger an increase in the expression of Tim-3 and PD-1 on T cells, we performed experiments to observe the functional consequences of this alteration.
These observations naturally lead to the notion of perturbations or knockouts: To understand what a specific element (gene, enzyme, neuron, brain area) does, lesion it and observe the functional consequences.
In a previous study [13] we observed that AE1 phosphorylation was apparently more pronounced and long standing in G− RBCs than in control RBCs following their treatment with diamide but, after a short incubation time, we did not observe any functional consequence.
Because of their transparency, severe morphological abnormalities in cardiac development are easily observed, but the functional consequences of these defects are difficult to quantify.
In the realistic Task 2, we have observed and analyzed the functional consequences, and thus inferred the functional roles, of the slow and fast dynamical components and of their interaction.
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