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From a comparison with calculated enthalpies obtained from density functional theory, we conclude that the experimentally observed destabilization originates from the well-mixed microstructure around the eutectic point.
The observed destabilization effect due to a large free volume is in agreement with previous observations.
These two factors, which are ultimately explained with a change in occupancy for conformers of specific side chains, may contribute to the observed destabilization.
The observed destabilization may reflect properties specific to NTS1: In contrast to NTS1, purification of an adenosine A2a receptor fusion protein by antagonist xanthine amine congener affinity chromatography with an estimated resin capacity of 14 mM did not lead to substantial amounts of A2a receptor protein in the antagonist column flow-through [23].
The observed destabilization of association between APPL1 and HDAC2-containing NuRD complex upon HDAC1 overexpression could explain the reduced nuclear localization of APPL1.
Since slowly exchanging amides are involved in the exchange of the aforementioned elements, the observed destabilization can be interpreted as an increase in structural dynamics.
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We did not observe destabilization of β-actin, suggesting that these proteins were specifically degraded by proteasomes or particular protease pathways.
As we did not observe destabilization of the GFP-cODC1-TDegF-RFP tester protein without TEV protease cleavage, this decrease might be caused by the leakiness of the GAL1 promoter [ 53] resulting in weak expression of the TEV protease.
Note that the weaker interactions that we observed between Hausp and Cry2 or hybrid constructs containing the C-terminus of Cry2 compared to those containing the Cry1 C-terminus are likely artefacts of overexpression in 293T cells since we did not observe destabilization of Cry2 upon Hausp depletion.
The presence of 2 μM α-Syn corresponds to a v-vesicle-lipid to α-Syn-protein ratio of 11 1, with the actual lipid to protein ratio being larger due to the presence of the saturated t-vesicle surface; at this condition, we did not observe destabilization of the v-SNARE vesicles by α-Syn since no leaking of content dyes from docked v-vesicles was observed.
It is observed that destabilization of colloidal asphaltene in oil production systems may occur if the surface charge and the resulting attraction forces between adsorbed resins and asphaltenes are disturbed (Leontaritis 1989; Alkafeef 2001).
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