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Kaur et al. (2013) observed amplification of carbon ion radiation in tumour cells (HeLa) loaded with gold nanoparticles (Kaur et al. 2013).
In most of these reactions, we observed amplification only of the appropriate family member matching the specific primer, indicating that this qRT-PCR platform detects microRNA with accuracy at the single nucleotide level (Figure 1B).
No inhibition was observed (amplification efficiency 2.0).
We observed amplification of mtDNA (MT-CO1) in both bisulfite-treated platelet mtDNA and leukocyte DNA.
However, we have observed amplification of CD29, CD73 and CD90 in oBM-MSCs, as well as the lack of CD34 and CD45 expression (unpublished work from our group).
Ried et al (1994) studied primary SCLC tumours and observed amplification of 1p32 in two of 13 cases (Ried et al, 1994).
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Genomic rearrangements lead to recurrent structural breakpoints within TERT promoter region, which correlates with highly elevated TERT expression and manifestation of kataegis, representing a mechanism of TERT upregulation in cancer distinct from previously observed amplifications and point mutations.
Our current study used SDs in the human chromosome 22q11.2 as reference, and thus sequence amplifications occurring specifically in the chimpanzee, gorilla and macaque genomes were not analyzed, but previous studies have observed amplifications specific to those lineages [ 24, 29].
They observed PCR amplification of CyD3.2 and CycD3.3, while no amplification of cycA.1, CycB1.1 and CycD3.1 was observed.
Tumour specimens were analysed for EGFR amplification in 20 patients, but no statistically significant correlation was observed between amplification status and response.
No adverse effect of heat treatment, processing conditions and ingredients was observed on amplification pattern.
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