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Pro 213 (TM V) is not accessible to Ile 193 (TM IV) in this conformation, and its observed accessibility in the presence of the agonist serotonin requires a conformational change.
One of these, the PPII-binding WW domain protein FE65 is thought to specifically recognize this particular Q/P junction, which would require the observed accessibility (i.e., solvent exposure) of said junction.
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Using this assay we reproducibly observed high accessibility to the BglII site in ARS1 in wt cells (Fig. 5A, lane 2), while the parallel samples from Δgcn5 cells (Fig. 5A, lane 5) consistently showed moderate accessibility.
Comparing Tables 4 and 5, we can observe that accessibility and ridership increase simultaneously, which is consistent with other studies [11].
Such inherent technical limitation of any mutational study is evident from the lack of epitope accessibility observed in studies with anti-IL11 antibodies (Figure 3B and data not shown).
The "harvest all" mentality is usually observed due to accessibility of the fishing grounds and landing areas.
We observed that chromatin accessibility is dramatically altered at the regions of H3K9me2 nucleation.
Similar to the variations observed in MNase accessibility, this differential effect in expression in immature ears spanned both arms of all chromosomes.
Instead, we suggest that at least some of the increased accessibility observed by Kondilis-Mangum et al (2010) could be explained by the eviction of H2A/H2B dimers.
Transcription transiently evicts H2A/H2B dimers, releasing 35 40 bp of nucleosomal DNA, and we demonstrate that H2A/H2B loss can explain the RSS accessibility observed in vivo.
We tested whether the enhanced chromatin accessibility observed at sites of H2A.Z and H3.3 deposition was reduced upon depletion of their respective loading factor, and found that the elevated accessibility we observed within regions of H2A.Z and H3.3 enrichment was partially lost upon Ep400 KD or Hira KD, respectively.
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