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They observed a higher cell viability and multilineage differentiation of the encapsulated hTMSC in the scaffolds.
Güven et al. [ 86] observed a higher cell yield with the Sepax 2 isolation system compared to standard manual isolation (2.6 ± 1.2 × 10 vs. 1.6 ± 0.9 × 10 nucleated cells/ml of liposuction) and 24%% higher clonogenicity.
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Because basonuclin's role in rRNA synthesis could only be observed at a higher cell density, all further analyses (e.g., IP or ChIP) were carried out at a high cell density of 4∼5×105.
Additionally, from the total number of differentiated cells we observed a higher rate of cells with fiber outgrowth at 6 weeks 90% as compared to cells with fiber outgrowths at 3 weeks 56%, in the BDNF-treated groups, indicating that these cells appear to be maturing over time.
In addition to decreased cell growth, we observed a higher percentage of cells with apoptotic nuclear morphology after suppression of DDX10 expression in these MCF-7 (95 apoptotic nuclei in DDX10-suppression vs. 22 in control, per 1000 cells) and MCF-10A (66 nuclei in DDX10-suppression vs. 34 in control, per 1000 cells) cell line.
In addition, we observed a higher cancer stem cell marker, CD44, and upregulation of epithelial mesenchymal transition (EMT) markers, including Zeb-1, Zeb-2, Twist, Slug and Dab2, compared with cells with epithelial cobblestone appearance.
Some authors have observed a higher rhythm of cell division in vitrified/warmed embryos compared with frozen/thawed embryos and this may impact the window of implantation and the length of progesterone treatment needed to synchronize the endometrium (Cercas et al., 2012).
We noted that with probes to the most 5' region of the transcribed ORG domain we observed a higher proportion of cells in early S phase compared to cells with middle or late PCNA patterns.
We further observed a higher percentage of cells with disrupted MMP and a higher rate of apoptosis in the cells expressing the Pro143Ala variant compared to wild-type protein.
We observed a higher proportion of cells in S-phase in those lines expressing EWS/WT1-KTS or EWS/WT1 + KTS compared to eGFP controls four hours following radiation.
We observed a higher number of cells with aberrant mitotic morphology and higher number of GMCs also in the tumour samples whose trastuzumab-treatment were changed to T-DM1 (P < 0.05).. Apoptotic cells were detected using CytoDeath staining, which localizes a caspase-activated breakdown product of cytokeratin subtype 18.
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