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We also observed a higher affinity for C∶C and bulge containing DNA compared to linear duplex and G∶T containing DNA.
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In the present study, we observed a high affinity amino acid permease AGP2 (MGG_13334.6) and an amino acid permease GAP1 (MGG_07606.6) among the differentially expressed genes.
Hajduk et al. observed a high correlation between the experimental NMR hit rate and the ability to bind drug-like small molecules with high affinity.
Suc-LLVY-MCA is mainly hydrolysed by the β5/β5i subunits (Mishto et al., 2014), and we observe a higher active site affinity (K aS ) with a lower Hill coefficient (n a ) in i- than in s-proteasomes; similarly i-proteasomes have a lower inhibitory site affinity (K iS ) with a higher Hill coefficient (n i ) compared to s-proteasomes.
Once again gefitinib exposure results in a higher affinity, observed as a higher saturation of the receptors and, most of all, a slower dissociation.
In pull-down assays we observed that curcumin seemed to have a higher affinity for the 130 kDa form of Cdc27.
A higher affinity is also observed in the curvature itself, with a slower dissociation.
This is observed for the NHX family in which AtNHX1-4 havequalaffinitiesies towards Na+ and K+, while AtNHX5 and 6 have a higher affinity towards K+.
AdipoR1 has a higher affinity for HMW.
As previously observed, 5aCON did not have a high affinity for PD 2a) compared to PD (Fig. 5B, compare lanes 2 and 6).
We focused on C. textile venom, as full competition with I-Bgt was observed, indicating the presence of a high affinity or abundant ligand.
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