Sentence examples for observe the localization from inspiring English sources

Each of the two genes was fused to the GFP and then transfected into Arabidopsis protoplasts to observe the localization of the GFP fusion proteins.

However, we did observe the localization of hGBP to the inclusion, and we speculate that this localization mark the inclusions for interaction with degradative compartments of the host cell.

To observe the localization of GFP-GAPA during cytokinesis, cells were first seeded on glass coverslips and synchronized using nocodazole (10 µM/ml) for 3 hrs to block cell division in mitosis.

To confirm the epifluorescence results and ensure that truncation of the protein was not responsible for the localization pattern, immunofluorescence microscopy was used to observe the localization of full-length AhpC with an M2 epitope at the C terminus.

The fusion gene and GFP control driven by CaMV 35S promoter were transformed into Arabidopsis protoplasts to observe the localization of GT-4.

B1647 cells, in the presence or absence of human serum (HS) and/or CD, were lysed by TX-100, centrifuged, and the supernatants were collected then separated by sucrose density-gradient (5 40%) centrifugation as described in Section 2. Nine fractions were collected and SDS-PAGE followed by Western blotting was performed to observe the localization of VEGFR-2.

In addition, we also directly observed the localization of NOC4L in Hela cells with the antibody against NOC4L by immunofluorescence assay (Fig.1F).

We more accurately determine the cellular distribution of this protein by observing the localization of an untagged Yer067w protein.

To establish the morphology of subcellular localizations by this approach, we first observed the localization of five marker proteins: cytosolic enolase, two proteins from the endoplasmic reticulum (Hsp70 and protein disulfide isomerase 5), mitosomal Hsp70 and glutaredoxin (Fig. 4A).

In addition to cell number, we also observed the localization of the ICM in blastocysts using a 3D imaging system (Fig. 3k and l and Movie S2 and S3).

Since the results obtained, by labelling cells with the PKCδ antibody or by observing the localization of PKCδ-GFP, were similar, the comparison between localization of PKCδ and PKCδ-DN, at the time in which the kinase moves to the nucleus, was done using both plasmids carrying GFP.