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Many Emergency Department patients with acute rhabdomyolysis are admitted to hospital to observe for development of renal failure.
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Wells were observed for development of cytopathic effect.
Animals were closely monitored and observed for development of disease at least twice daily.
In addition, the 10-fold dilutions were incubated at 37°C for 14 days, then observed for development of a color change indicating growth.
siRNA transfected cells (control or Src+STAT3 siRNA) were implanted into the mammary fat pad (4 mice/group) and the mice were observed for development of palpable tumors at the site of implantation.
All subjects were observed for development of diabetes to the end of 2007.
Cells were observed for development of the blue coloration with a microscope at a magnification of 400×.
At the end of the exposure period, residual test substance was removed and the animals were observed for development of any adverse skin reactions daily for 14 days.
Nonfeeding ticks were kept at 85% relative humidity, 20° 22°C, natural photoperiod (Hamilton, MT), and observed for development in their life cycle.
(B ) Fibroblast cells were observed for development of cytopathogenic effect by bright-field microscopy at 12, 24 and 36 hr post-infection.
The data demonstrate translational importance since similar intra-individual trends were observed for development of RR in different endemic areas, allowing global application of these biomarkers in tests for early diagnosis of RR.
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