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New South Wales, Australia Neww drivers display 'P' plates for 24 months, observe extra speed limits and zero alcohol.
In the presence of 10 μM human Trx we observe extra steps following the unfolding of the CD4 domains.
We did not directly observe extra rounds of templated centriole replication in Ubq-GFP-Sak embryos, but we suspect that this is because the protein was expressed at very low levels in embryos.
We did not observe extra density for MBP when fused to the N-terminus of full-length Orc1 or Orc2, nor did we see GFP fused to the C-terminus of Orc2 and Orc4 (data not shown), suggesting these protein termini are flexible.
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More interestingly, we observed extra density on the degenerate site of SUR1 NBD1.
Consistent with this, we observed extra lag-2 GFP-strong-positive celag-2 GFP-strong-positivep/Z4.a-derived celag-2 GFP-strong-positive the gonad) in cki-1(RNAi) animalag-2 GFP-strong-positive
We also observed extra high molecular weight bands in the offspring of F0 #31 (Fig 3c).
We observed extra CCAP-ENs in several abdominal segments, a phenotype not observed in control larvae incubated in PBS.
Unexpectedly, we also observed extra Crz-immunoreactive (ir) neurons located laterally to the vCrz neurons (Fig. 5A; Table 3).
I also observed extra bands in the 3xFLAG lines for NHR-23 and NHR-25 compared to the corresponding 2×FLAG lines, which would be consistent with NHR-23 and NHR-25 isoforms, though they could also represent degradation products.
Indeed, that would mean that the actual number of mutations that have reached full expansion at any age is higher than observed (extra mutations being those that had died), implying that mutations expand faster than it appears.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com