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In order to obtain an amplification of the degradation of the mRNA and to observe a phenotype in rice leaves, it might be necessary to increase the level of agro-infection in rice tissues.
We did not observe a phenotype specific to Δ256Foxo1AGRP mice.
We did not observe a phenotype associated with individually knocking down aakb-1 and aakb-2.
Transgenic over-expression of human mutant genes has been extensively used to generate mouse models for human neurodegenerative disorders due to the need of over-expressing enough amount of the mutant protein in order to observe a phenotype.
While Roc1a does not bind Cul5 when Roc2 protein is present, it is possible that we were unable to observe a phenotype in Roc2 mutants because Roc1a can bind Cul5 in the absence of Roc2, and thereby functionally substitute for Roc2.
However, we do observe a phenotype that casts doubts on a complete loss of function.
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We have also observed a phenotype involving development of the tail and trunk (Figures 5I and 5J).
Genetic or functional redundancy is another possibility for not observing a phenotype after silencing.
Indeed, we have observed a phenotype consistent with this in our study following Stat1 and Stat2 suppression using siRNA.
We never observed a phenotype in Smed-mll5-1 RNAi), Smed-mll5-1 RNAiding Smed-mll5-1 RNAievento nine feedings.
Interestingly, we observed a phenotype similar to that of the Lis1 knockdown in the brains of Dpy19l1 knockdowns (Fig. 5).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com