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The PCR conditions were as follows: 5 min of initial enzyme activation at 95°C, 45 cycles of denaturation at 95°C for 10 s, annealing at 60°C for 20 s and elongation 72°C for 30 s. Subsequently, melting curves were obtained by measuring the drop in fluorescence when raising the temperature from 67 to 98°C with 5 observations per second.
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In the rising phase of the microwave burst, a non-thermal gyrosynchrotron source was observed by the high-rate (10 images per second) observations to propagate from one end of the loop to the other with a speed of 9 × 104 km s−1.
GPS observations with higher than once-per-second sampling are becoming increasingly important for seismology.
After allowing the fish at least 3 min to get used to the conditions inside the chamber, they were filmed for 10 min with a video camera located approximately 1 m from the observation tank at 30 frames per second.
Observations were recorded at 12.5 frames per second and analysed at 6.25 frames per second.
"The queries are long-running and the data changes many times per second as new records, observations, or log entries arrive.
The observation was undertaken by video-recording (30 frames per second) the tool-use of the adults in the area of the shrine.
The average number of overdue temperature observations per day was 1.4 in the first period and 0.9 in the second (P < 0.01).
Mice demonstrating more than 1 × 10 photons per second or reaching end of the observation periods are euthanised.
Observations by Galileo suggest lava coverage rates at Pillan between 1000 and per second during the 1997 eruption.
Real-time observations suggest DNA rotation during Holliday junction branch migration at 1.6 revolutions per second [ 76].
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