From observations of mutant mice, deletion of canonical Wnt/ β-catenin pathway leads to the defect of osteoblastogenesis both in endochondral and membranous ossification [ 3, 4].
Previous studies have been done in reconciling differences between in silico prediction and in vivo observations of mutant phenotypes [ 17, 18, 31].
Despite these differences in biochemistry, there was no significant morphological or developmental change in the TrCel5AER plants, which is consistent with previous observations of mutant plants with changes in the XyG side chain composition [ 57].
Observations of p5cs-1 mutants revealed that application of ANE and LPC did not alter the sensitive phenotype of p5cs-1 mutants.
HS-AFM observations of activity mutants clearly demonstrated that conformational dynamics of TClpB is linked to the disaggregation activity.
Observations of xylp mutants hinted that rice XYLP genes may have a function in the development of organs with vascular systems.
This result is in agreement with previous observations of Ngb mutants mentioning that H64Q and H64L/K67L were "remarkably stable in the oxygenated form".
Based on previous observations of Sui− mutants, it is possible that the Rps5 substitutions do not elevate the UUG AUG ratio sufficiently to produce enough his4-301 product for adequate histidine biosynthesis (Dorris et al., 1995; Martin-Marcos et al., 2013).
Although we did not conduct experiments addressing a possible link between the exocyst and ABCB auxin transporters, mutation of ABCB transporters leads to phenotypes not consistent with our observations of exocyst mutants (e.g., longer root hairs [ 91, 93], enhanced gravitropism [ 94, 95], sporadic root curvature [ 94], prominent reduction of the number and growth of lateral roots [ 94, 96, 97]).
Iris phenotypes of the single-congenic strains closely resembled our previous observations of mice mutant for only Gpnmb or Tyrp1 in varying genetic contexts [ 22, 23, 36].
Observations of these mutant phenotypes have led to the speculation that Brat may function to inhibit cell growth in one of two newly born neuroblast daughters so as to generate one neuron and one neuroblast, rather than two neuroblasts [ 32].
